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Preparation of polyvinylpyrrolidone/cadmium sulfide quantum dots modified electrode and its determination of hemoglobin

wallpapers News 2021-11-23
Preparation of polyvinylpyrrolidone/cadmium sulfide quantum dots modified electrode and its determination of hemoglobin
Prepared polyvinylpyrrolidone (PVP) surface-modified cadmium sulfide (CdS) semiconductor nanocrystals (quantum dots) and modified glassy carbon electrodes for the study of the electrochemical behavior of hemoglobin (Hb). The experimental results show that Hemoglobin has a good current response on the modified electrode, and the flow injection analysis results further show that the modified electrode has good stability and reproducibility. In the concentration range of 1.0×10-8~2.0×10-5mol/L, The concentration of hemoglobin has a good linear relationship with its response current, the linear correlation coefficient is 0.9986, and the detection limit is 5.0×10-9mol/L. This method was used for the determination of hemoglobin in whole blood, and good results were also obtained.
In this paper, a sol-gel method was used to prepare polyvinylpyrrolidone (PVP) surface-modified cadmium sulfide (CdS) semiconductor quantum dots, which were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), and other means; the modification was performed A quantum dot modified electrode was fabricated on the surface of a glassy carbon electrode (GC), and the electrochemical behavior of myoglobin (Myoglobin, Mb) on the quantum dot modified electrode was studied. The experimental results showed that the PVP/CdS quantum dot modified electrode pair Myoglobin has a catalytic reduction effect, and the reduction peak current has a good linear relationship with the measured protein concentration. The flow injection analysis results further show that the quantum dot modified electrode has high stability and good reproducibility, and the detection limit is 2.0 ×10.mol/L, the electrode can be used as a new high-sensitivity electrochemical sensor for detecting myoglobin.
Using carbon ionic liquid electrode (CILE) as the working electrode, after fixing BGQDs on the surface of CILE, Hb was fixed on the surface of the electrode with Nafion membrane to prepare modified electrodes (Nafion/Hb/BGQDs/CILE). By Fourier infrared, The transformation spectrum proved that Hb still maintains the original protein secondary structure on the surface of the BGQDs modified electrode. The electrochemical behavior of the modified electrode in a phosphate buffer solution at pH 4.0 was studied by cyclic voltammetry, and a pair of peak-shaped symmetric redox peaks appeared. It shows that BGQDs have a good promotion effect on the direct electron transfer of Hb. The direct electrochemical behavior of Hb is further studied, the electrochemical parameters are solved, and the electrocatalytic behavior of the modified electrode on sodium nitrite is explored, and the apparent Michaelis The constant is 6.372 mmol L.
 

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